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Nature Article

New Nature Paper: ecNGS for genomic safety

Read the Nature paper
TwinStrand Biosciences
  • Technology
  • DuplexSeq™ Kits
    • AML Assay
    • Mutagenesis Assay
    • Custom
  • Resources
    • Publications
    • Posters
    • Safety
    • Patents
  • Company
    • Our Team
    • Careers
    • Events
    • News
    • Investors
  • Contact

 

TwinStrand Duplex Sequencing™ Technology

 

Download the brochure

Duplex Sequencing is an ultra-high accuracy sequencing method that overcomes the limitations of Next-Generation Sequencing (NGS) by independently tracking both strands of individual DNA molecules. The paired sequences are compared to eliminate errors.

Duplex Sequencing Reveals True Mutations

 

  • > 10,000-fold increase in accuracy over standard NGS
  • Eliminates virtually all background errors, revealing ultra-low frequency variants
  • Compatible with any Illumina® sequencer
  • Easy to adopt with no special equipment needed

 

 

The same gene sequenced by standard Illumina® sequencing (left) and with Duplex Sequencing (right). With conventional methods, every position in the gene appears mutated in 0.1 – 1% of molecules sequenced. Duplex sequencing eliminates the background noise, revealing the previously hidden true mutation. 

Duplex Sequencing Eliminates Technical Noise

 

  • Duplex Sequencing capitalizes on the naturally-occurring complementarity between strands of the DNA double-helix
  • Each strand of each original molecule is uniquely labelled such that both can be tracked throughout amplification and sequencing for subsequent error correction

Brochure TwinStrand Duplex Sequencing Technology

See how it works

Our Solution

TwinStrand Duplex Sequencing products offer the greatest accuracy in DNA sequencing available today. Our kits include everything needed to convert DNA into Duplex Libraries, ready to run on any Illumina® sequencer. The robust 2 – 3 day hybrid capture-based workflow is familiar to and easily integrated into existing workflows.

 

Our platform includes access to a secure cloud-based bioinformatics pipeline. The pipeline is expertly tuned and optimized for accurate, easy-to-use, primary and secondary analysis.

TwinStrand Duplex SequencingTM

You bring:

 

  • DNA sample
  • Water and ethanol

 

We provide library prep kit:

 

  • Duplex-optimized library preparation reagents
  • DuplexSeqTM adapters
  • Dual unique indexes
  • PCR reagents
  • Hybrid capture reagents
  • Magnetic beads
  • TwinStrand DNA standards

Multi-box configuration facilitates best practices, separating pre- and post- PCR protocol steps.

 

You bring:

 

  • Illumina® sequencer

     

     

We provide bioinformatics solution:

  • Purpose-built for DuplexSeq data
  • Easy-to-use, secure cloud-based software hosted on DNAnexus®
  • Processes raw read files to produce duplex error-corrected sequences, variant calls, and assay quality metrics
  • Industry standard formats compatible with existing annotation

Inputs: demultiplexed FASTQ files from any Illumina® platform

 

Outputs: error corrected BAM, VCF, and CSV files

Request a Quote

Explore Applications

Explore Applications and Publications

 

 AML MRD  

    Mutagenesis    

Cellular Immunotherapy

Collaboration

Publications

Want to learn more or try TwinStrand Duplex Sequencing technology?

Contact us

 

TwinStrand Biosciences, Inc.
3131 Elliott Ave, Suite 750 • Seattle, WA 98121 • USA
+1 (877) 202-TWIN

 

For Research Use Only. Not for use in diagnostic procedures.

©2023 TwinStrand Biosciences, Inc. All rights reserved. All trademarks are the property of TwinStrand Biosciences, Inc. or their respective owners.  Legal Notices

  




American Society of Hematology (ASH)

December 5-8, 2020 – Virtual

 

Poster Presentation

 

“Duplex Sequencing with Patient-Specific Hybrid Capture Panels Reveals Ultra-Low Frequency Measurable Residual Disease in Pediatric Acute Myeloid Leukemia”

 

Presented by Jake Higgins, Senior Scientist – December 6, 2020 7:00 a.m.-3:30 p.m. PST

 

Conference Website

 

Society of Toxicology (SOT)

March 10-14, 2019 – Baltimore, MD

 

Poster Presentations

 

 

View Poster

 

 

Conference Website

 

AACR Annual Meeting – San Diego, CA

March 29-April 3, 2019

 

Poster Presentations

 

 

View Poster

 

 

View Poster

Presentations by CEO Jesse Salk, MD, PhD – AGBT 2020

Ultra-Sensitive Residual Leukemia Detection with Patient-Specific Dupex Sequencing