Duplex Sequencing^TM Technology

Duplex Sequencing is an ultra-high accuracy sequencing method that overcomes the limitations of Next-Generation Sequencing by independently tracking both strands of individual DNA molecules. The paired sequences are compared to eliminate errors.

Duplex Sequencing Reveals True Mutations

> 10,000 fold increase in accuracy over standard NGS
Eliminates virtually all background errors, revealing ultra-low frequency variants
Compatible with any Illumina^® sequencer
Easy to adopt with no special equipment needed

The same gene sequenced by standard Illumina^® sequencing (left) and with Duplex Sequencing (right). With conventional methods, every position in the gene appears mutated in 0.1 – 1% of molecules sequenced. Duplex Sequencing eliminates the background noise, revealing the previously hidden true mutation.

Duplex Sequencing Eliminates Technical Noise

Duplex Sequencing capitalizes on the naturally-occurring complementarity between strands of the DNA double-helix
Each strand of each original molecule is uniquely labelled such that both can be tracked throughout amplification and sequencing for subsequent error correction

Our Solution

TwinStrand’s Duplex Sequencing product offers the greatest accuracy in DNA sequencing available today. Our kits include everything needed to convert DNA into Duplex Libraries, ready to run on any Illumina^® sequencer. The robust 2 – 3 day hybrid capture-based workflow is familiar to and easily integrated into existing workflows.

Our platform includes access to a secure cloud-based bioinformatics pipeline. The pipeline is expertly tuned and optimized for accurate, easy-to-use, primary and secondary analysis.

TwinStrand Duplex Sequencing

You bring:

DNA sample
Water and ethanol

We provide library prep kit:

Duplex-optimized library preparation reagents
DuplexSeq^TM adapters
Dual unique indexes
PCR reagents
Hybrid capture reagents
Magnetic beads
TwinStrand DNA standards

Multi-box configuration facilitates best practices, separating pre- and post- PCR protocol steps.

You bring:

Illumina^® sequencer

We provide bioinformatics solution:

Purpose-built for DuplexSeq data
Easy-to-use, secure cloud-based software hosted on DNAnexus^®
Processes raw read files to produce duplex error-corrected sequences, variant calls, and assay quality metrics
Industry standard formats compatible with existing annotation

Inputs: demultiplexed FASTQ files from any Illumina^® platform

Outputs: error corrected BAM, VCF, and CSV files