• Technology
  • DuplexSeq™ Kits
    • AML Assay
    • Mutagenesis Assay
    • Custom
  • Resources
    • Publications
    • Posters
    • Safety
    • Patents
  • Company
    • Our Team
    • Careers
    • Events
    • News
    • Investors
  • Contact

On-demand webinar

The future of mutagenesis testing is here.

Watch now
TwinStrand Biosciences
  • Technology
  • DuplexSeq™ Kits
    • AML Assay
    • Mutagenesis Assay
    • Custom
  • Resources
    • Publications
    • Posters
    • Safety
    • Patents
  • Company
    • Our Team
    • Careers
    • Events
    • News
    • Investors
  • Contact

 

Mutagenesis Detection & Analysis Assay

 

Mutagenesis is an important predictor of cancer risk in chemical and drug safety testing. Standard mutagenesis assays yield a limited amount of indirect information about a compound’s mutagenic potential and/or are complex and cumbersome.

 

TwinStrand DuplexSeq™ Mutagenesis Assays offer a fundamentally new Next-Generation Sequencing (NGS)-based approach for mutational research that directly detect, quantify, and characterize induced mutagenesis in the DNA of exposed organisms and cells. The unprecedented richness of data unlocks powerful opportunities for novel insights and discoveries.

 

  • The first NGS technology capable of directly measuring mutagenesis in DNA molecules without the need for biological selection
  • Accurately & reproducibly measures mutation loads on the order of 1/10,000,000
  • Equally applicable in vitro and to any tissue in vivo
  • Produces comprehensive mutation data, including frequency, simple spectrum, and trinucleotide signature
  • Supports the diverse needs of academic, commercial, and regulatory users
  • DuplexSeq™ Mutagenesis Assays currently available in human, mouse, and rat formats

 

Download Data Sheet

Mutation Frequencies by Treatment Group

 

 

Urethane-induced mutagenesis detected by duplex sequencing in 3 mouse tissues. Mutation frequencies are highly reproducible across biological replicates (n=5/group), and statistically robust. The number of individual mutant nucleotides identified per sample among hundreds of millions screened is indicated above each bar. VC: vehicle control (untreated).

 

Download Data Sheet

Panel Overview

The TwinStrand Duplex Sequencing mutagenesis kits use a 48kb panel targeting 20 arbitrary regions spread throughout the genome. The regions are balanced to provide an unbiased sampling of representative sequence contexts throughout the genome (GC content, genic/non-genic, coding/non-coding, etc). These sites have no known role in cancer and are unlikely to be significantly influenced by positive or negative selection. Currently available for human, mouse, and rat studies.

 

 

Location of genomic targets in the TwinStrand Duplex Sequencing rat mutagenesis panel. Mouse and human panels are similarly constructed.

 

Download Data Sheet

Simple Mutation Spectra

 

 

Simple mutation spectra detected by duplex sequencing of urethane-exposed mouse tissue. Unsupervised hierarchical clustering perfectly assigned all of the 30 tissues to treatment groups (vehicle control on left, urethane on right).

 

Request a Quote

Trinucleotide Mutation Signatures

 

 

Highly distinct trinucleotide signatures generated by duplex sequencing of normal (non-tumor) mouse tissues exposed to three mutagens: urethane, benzo[a]pyrene, and N-ethyl-N-nitrosourea. Figures generated using ultra-low frequency mutations present on the order of 1-in-1 million.

 

Request a Quote

Scientific Content

 

POSTER: Detection of Ultra-rare Mutations in vivo Establish biomarkers of Endogenous and Environmental Exposure

Poster: Duplex Sequencing Detects Rare Subclonal Variants that Mark Early Carcinogenesis and Preneoplastic Clonal Evolution

POSTER: Non-invasive Detection of Aristolochic Acid Exposure Using Ultra-Sensitive Duplex Sequencing

PAPER: Next-Generation Genotoxicology: Using Modern Sequencing Technologies to Assess Somatic Mutagenesis and Cancer Risk

Download the TwinStrand Duplex Sequencing Mutagenesis brochure

TwinStrand DuplexSeq™ 
Mutagenesis Assays

Download the Mutagenesis Key Publications Sheet

TwinStrand Mutagenesis Key Publications Sheet

Download the TwinStrand Duplex Sequencing Mutagenesis Sheet

TwinStrand DuplexSeq Mutagenesis Assays

 

TwinStrand Biosciences, Inc.
3131 Elliott Ave, Suite 750 • Seattle, WA 98121 • USA
+1 (877) 202-TWIN

 

For Research Use Only. Not for use in diagnostic procedures.

©2022 TwinStrand Biosciences, Inc. All rights reserved. All trademarks are the property of TwinStrand Biosciences, Inc. or their respective owners.  Legal Notices

  




American Society of Hematology (ASH)

December 5-8, 2020 – Virtual

 

Poster Presentation

 

“Duplex Sequencing with Patient-Specific Hybrid Capture Panels Reveals Ultra-Low Frequency Measurable Residual Disease in Pediatric Acute Myeloid Leukemia”

 

Presented by Jake Higgins, Senior Scientist – December 6, 2020 7:00 a.m.-3:30 p.m. PST

 

Conference Website

 

Society of Toxicology (SOT)

March 10-14, 2019 – Baltimore, MD

 

Poster Presentations

 

 

View Poster

 

 

Conference Website

 

AACR Annual Meeting – San Diego, CA

March 29-April 3, 2019

 

Poster Presentations

 

 

View Poster

 

 

View Poster

Presentations by CEO Jesse Salk, MD, PhD – AGBT 2020

Ultra-Sensitive Residual Leukemia Detection with Patient-Specific Dupex Sequencing